Unlock Deep Sleep: Discover The Best Amino Acids for Restful Nights

Amino acids, the building blocks of proteins, play a crucial role in many biological processes, including those that directly and indirectly affect sleep. These versatile compounds are more than just nutrients; they are precursors to neurotransmitters, hormones, and other bioactive molecules that regulate the sleep-wake cycle, stress response, and muscle relaxation. 

From the calming effects of Glycine to the serotonin-boosting capabilities of L-Tryptophan, amino acids offer a range of benefits that can significantly improve sleep quality. Different amino acids target various pathways involved in sleep. For instance, L-Theanine increases the production of alpha brain waves, promoting a state of relaxed alertness conducive to sleep

Restful Sleep With Amino Acids

On the other hand, GABA acts as an inhibitory neurotransmitter, reducing neuronal excitability and paving the way for a peaceful night's rest. Understanding the unique roles that each amino acid plays in sleep regulation can create a targeted approach to supplementation that addresses specific sleep concerns. Whether you struggle with falling asleep, staying asleep, or achieving restorative, deep sleep, an amino acid can likely help.


Glycine is a non-essential amino acid that has garnered attention for improving sleep quality. It works primarily by lowering your body temperature, a natural signal to the body that it's time to wind down and prepare for sleep—additionally, glycine aids in the production of serotonin, a neurotransmitter crucial for sleep regulation. 

Product Overview: Life Extension Glycine 1000 mg is a dietary supplement that supports relaxation, healthy sleep, and overall mental health. Glycine, a semi-essential amino acid, is known for its calming effect on the brain, making it a valuable aid for those looking to improve their sleep. 

This supplement offers a substantial 1000 mg dose to maximize its potential benefits, extending to mood enhancement, cognitive support, and maintenance of healthy glucose metabolism. Additionally, it may help preserve muscle mass and contribute to a nutritional body composition.

Studies have shown that supplementing with Glycine before bedtime can help you fall asleep faster and enjoy a more restful, deeper sleep. Therefore, Glycine can be a valuable addition to your sleep-enhancing supplement arsenal.


  • Provides a high dose of 1000 mg of Glycine for enhanced efficacy.
  • Promotes relaxation and healthy sleep patterns without causing daytime drowsiness.
  • It may support mood, cognition, and mental health.
  • It is involved in collagen production and supports healthy glucose and fructose metabolism.
  • Non-GMO, gluten-free, and manufactured in the USA with high-quality raw ingredients.


  • As a single amino acid supplement, it may not address all the complex needs of those with severe sleep disturbances.
  • Some users may prefer a lower dose or a blend with other sleep-supportive nutrients.Cons 1Cons 2Cons 3Cons 4

My Take: Life Extension's Glycine is an excellent choice for those seeking to naturally improve their sleep quality and mental well-being. Its higher dosage is scientifically grounded, suggesting it could offer more significant benefits than traditional glycine supplements. The emphasis on quality ingredients and manufacturing practices further assures its potential as a safe and effective supplement for enhancing overall health.

Glycine really is the top supplement of choice for lowering your body temperature and thus signaling to your body that it is time to go to sleep.


GABA, or Gamma-Aminobutyric Acid, is a neurotransmitter that plays an important role in calming neurons in the brain. By binding to specific receptors, GABA promotes relaxation, reduces mental and physical stress, and makes it easier for the mind and body to transition into sleep mode. 

Supplementing with GABA has been shown to help individuals fall asleep faster and experience more profound, more restorative sleep stages. However, it's worth noting that not all GABA supplements cross the blood-brain barrier effectively, so choosing a high-quality supplement is crucial for achieving the desired effects on sleep.

Product Overview: Double Wood's GABA supplement contains 300 capsules, each offering a substantial 1,000mg of GABA. This neurotransmitter is essential for brain function and is often supplemented to support relaxation and better sleep. Made in the USA, the product is vegan, gluten-free, and non-GMO, making it accessible to various dietary needs.


  • High-dose GABA promotes relaxation and supports healthy sleep. Provides a high dose of 1000 mg of Glycine for enhanced efficacy.
  • The product is vegan, gluten-free, and non-GMO, catering to various health-conscious consumers.
  • They are manufactured in the USA, ensuring quality and safety standards.


  • GABA's efficacy can vary significantly among individuals, with some users not experiencing the desired effects.
  • Potential side effects and interactions with other medications, so it is important to check with your healthcare provider before taking them.

My Take: Double Wood's GABA is an excellent choice for those seeking natural support for relaxation and sleep. Its high dosage and the product's purity are impressive, but as with any supplement, it's essential to consider individual responses and potential interactions with other medications. 

Consulting with a healthcare professional before starting GABA is advisable, especially for those with pre-existing health conditions or those currently taking other medications.


5-HTP, or 5-Hydroxytryptophan, is a naturally occurring amino acid precursor to serotonin, a neurotransmitter essential for mood regulation and sleep. When serotonin levels are balanced, they can be more effectively converted into melatonin, the hormone responsible for regulating the sleep-wake cycle. 

Supplementing with 5-HTP has been shown to improve the duration and quality of sleep by extending the REM phase, allowing for a more restorative rest. However, it's worth noting that long-term use of 5-HTP should be monitored closely, and it's advisable to consult a healthcare provider for personalized guidance, especially if you're taking other medications or supplements that affect serotonin levels.

Product Overview: Dr. Emil Nutrition's 5-HTP Plus supplement is crafted to support mood and rest. It combines 200 mg of 5-HTP with 20 mg of SAM-e to enhance the conversion and efficacy of 5-HTP, while Vitamin B6 is included to support this conversion process further. This blend promotes a calm and relaxed mood by optimizing the body's 5-HTP conversion.


  • Formulated with a synergistic blend of 5-HTP, SAM-e, and Vitamin B6 for optimal conversion and effectiveness.
  • It may aid in promoting a calm and relaxed mood by supporting natural neurotransmitter production.
  • Gluten-free, non-GMO, and produced in a certified cGMP facility.


  • The efficacy of the supplement may vary from person to person.
  • Individuals on medications or with pre-existing conditions should consult with a healthcare provider before use.

My Take: This supplement is a thoughtfully formulated product to support mood stabilization and restfulness. Including both SAM-e and Vitamin B6 with 5-HTP is a strategic choice that could enhance the overall effectiveness of the supplement, potentially making it a valuable addition to a wellness regimen and sleep supplementation plan.


Taurine is a sulfur-containing amino acid that plays a role in a variety of physiological processes, including neurotransmission and regulation of calcium levels in cells. It has a calming effect on the nervous system, which can be particularly beneficial when trying to wind down for sleep. 

Taurine facilitates the release of GABA, an inhibitory neurotransmitter that promotes relaxation and counters the effects of stress hormones like cortisol. Some studies suggest that taurine supplementation can improve sleep quality by enhancing the proportion of restorative, deep stages of sleep. Because of this, taurine is often included in sleep-promoting supplement formulations for its synergistic benefits.

Product Overview:

Life Extension's Taurine supplement provides a  1000 mg dose of one of the most essential amino acids in the body.  It supports heart health and nervous system function and may contribute to longevity and healthy aging. Taurine is also beneficial for athletes, aiding in muscle recovery post-exercise and helping to preserve muscle mass over time.


  • High-dose taurine supports cardiovascular and nervous system health.
  • It may contribute to longevity and muscle recovery, benefiting athletes and older adults.
  • The product is sourced from high-quality ingredients, non-GMO, and gluten-free.


  • Some individuals may require a lower dose, depending on their health status and dietary intake.
  • Taurine's broad range of benefits may overshadow its specific effects, making it less targeted than condition-specific supplements.

My Take: Life Extension's Taurine supplement stands out for its quality and concentration, offering a potent dose that can benefit various health needs, from athletic recovery to longevity. Its role in supporting cognitive and retinal health further broadens its appeal as a multi-functional amino acid.


L-tryptophan is an essential amino acid precursor to serotonin, a neurotransmitter key for mood regulation and sleep. Once in the body, L-Tryptophan is converted into 5-HTP, then turned into serotonin. This chain reaction culminates in the production of melatonin, the sleep hormone. 

Several studies indicate that supplementing with L-Tryptophan can improve sleep latency (the time it takes to fall asleep) and contribute to more restful sleep. Because it's a natural building block for sleep-related chemicals in the brain, L-Tryptophan is often considered a safe and effective supplement for those looking to improve their sleep quality.

Product Overview: Amazing Formulas' L-Tryptophan 1000mg Tablets are designed to naturally support sleep, mood enhancement, immune system function, and stress relief. This supplement provides a substantial dosage of L-tryptophan, an essential amino acid that plays a crucial role in the production of serotonin.


  • Offers a high dosage of 1000 mg for adequate support.
  • Supports multiple aspects of health, including sleep, mood, and immune function.
  • Free from added herbs and drugs, emphasizing safety for adult use.
  • Manufactured in the USA with adherence to Good Manufacturing Practices.


  • The large pill size and taste may only be suitable for some consumers.
  • As with any supplement, efficacy can vary between individuals.

My Take: Amazing Formulas' approach to providing a natural sleep aid and mood support through L-Tryptophan is impressive, considering the clean formulation and absence of additional herbs or drugs. This product could benefit those seeking a holistic option to improve their sleep quality and overall well-being.


L-Theanine is an amino acid commonly found in tea leaves and is well-known for its calming effects. It promotes relaxation without causing drowsiness by modulating inhibitory neurotransmitters, stress hormones, and brain wave activity. Specifically, L-theanine enhances the production of alpha brain waves, encouraging a state of relaxed alertness and making it easier to drift off to sleep. 

Studies suggest that when taken before bedtime, L-Theanine can improve sleep quality by helping you fall asleep more quickly and increasing the duration of deep sleep. As a result, it's a popular choice for those looking to improve both the quality and quantity of their sleep.Product Overview: The Double Strength L-Theanine Supplement by Sports Research, known as Suntheanine Softgels, is specifically formulated to enhance mental focus and calmness. Each soft gel contains 200mg of L-Theanine, designed to increase alpha brain waves for relaxation without the side effects associated with caffeine.


  • Contains a clinically researched, patented form of L-theanine.
  • Double the typical dose found in other supplements for enhanced support.
  • Includes 100% organic coconut oil, non-GMO, gluten-free, and soy-free ingredients.


  • Some individuals may be sensitive to the effects of L-Theanine or coconut oil.
  • The product is in soft gel form, which everyone may not prefer.

My Take: Sports Research's approach to L-theanine supplementation is commendable for its focus on quality and effectiveness. The use of patented Suntheanine suggests a commitment to providing a reliable, researched-backed product, making it an excellent choice for those seeking natural cognitive support and relaxation.


Arginine is an amino acid that plays multiple roles in the body, including the synthesis of proteins and the regulation of blood flow through nitric oxide production. While it's not directly linked to sleep, arginine has been studied for its potential effects on improving sleep quality indirectly. For example, increased blood flow can lead to better oxygenation and nutrient supply to tissues, which may contribute to a feeling of physical relaxation conducive to sleep. 

Research also suggests that arginine may help reduce stress by lowering cortisol levels, making falling and staying asleep easier. However, the evidence of arginine's impact on sleep is not as robust as other amino acids, so it's best to consult a healthcare provider for personalized advice.

Product Overview: NOW Supplements' L-Arginine tablets offer a potent 1,000 mg dose of this conditionally essential amino acid, known for its role as a nitric oxide precursor and its ability to aid in the dilation of blood vessels. This supplement is made with pharmaceutical-grade L-Arginine and adheres to non-GMO, Kosher, and soy-free standards.


  • Each tablet provides a double-strength dose (1,000 mg) of L-arginine.
  • Supports vascular health by aiding in blood vessel dilation.
  • Certified non-GMO, Kosher, and free from common allergens.


  • It may require caution and consultation with a physician, especially for those with certain medical conditions or other medications.
  • The high dose may be more than necessary for some individuals and could lead to side effects if not taken correctly.

My Take: The double-strength L-Arginine tablets from NOW Supplements are an excellent choice for those looking to support cardiovascular health through a nitric oxide-boosting supplement. With its rigorous manufacturing standards and quality assurance, this product is poised to deliver the promised health benefits effectively.


Ornithine is an amino acid that plays a role in the urea cycle, helping to rid the body of ammonia, a waste product of protein metabolism. While not directly a sleep-inducing amino acid, ornithine has been studied for its potential stress-reducing benefits, which in turn can have a positive impact on sleep quality. 

Research suggests that supplementing with ornithine can help alleviate feelings of stress and improve various markers of sleep quality, such as sleep duration and onset latency. By promoting a relaxed state and reducing physiological stress markers, ornithine can contribute to an environment that fosters better sleep.

Product Overview: Nutricost L-Ornithine capsules provide a substantial 500mg dose of L-Ornithine, an amino acid associated with stress relief and potentially aiding sleep improvement. The tablets are designed to be easy to swallow and are produced in a facility that complies with Good Manufacturing Practices, ensuring a high-quality, non-GMO, and gluten-free product.


  • Each capsule contains a significant 500mg dose of L-ornithine.
  • The product adheres to strict quality control measures, being non-GMO and gluten-free.
  • Manufactured in a GMP-compliant and FDA-registered facility.


  • The effects of L-ornithine may vary from person to person, with some individuals not experiencing the expected benefits.
  • Specific health conditions or medications may necessitate a consultation with a healthcare provider before use.

My Take: The Nutricost L-Ornithine capsules are a noteworthy option for those seeking the potential stress-relief and sleep-enhancing benefits of this amino acid. The commitment to quality and adherence to manufacturing standards make this a reliable supplement choice within the wellness market.

My Favorites



Why it's a top pick: Life Extension Glycine makes the list for its multi-faceted health benefits, including supporting metabolic health and potentially improving sleep quality. The reputable brand is known for its rigorous research and quality supplements, making this Glycine supplement a reliable choice for those looking to support their sleep naturally.



Why it's a top pick: Amazing Formulas L-Tryptophan is noteworthy due to its high potency and multifaceted benefits. As an essential amino acid, L-Tryptophan is a precursor to serotonin, a neurotransmitter crucial for maintaining a balanced mood and healthy sleep patterns. The 1000 mg dose in each tablet ensures that the body receives an adequate amount to influence positive changes in sleep quality. It's also free from added herbs and drugs, which is a pure way to supplement your diet with this vital nutrient. Additionally, L-Tryptophan has been shown to support immune system function and stress relief, rounding out its benefits for overall well-being.



Why it's a top pick: Sports Research's L-Theanine, featuring Suntheanine, is my go-to for a non-drowsy, relaxed state. It's perfect for those who want to wind down without the side effects of traditional sleep aids. Despite the current stock issues, the quality assurance that comes with a GMP-certified facility in the USA is a significant plus.


L – Ornithine

Why it's a top pick: Nutricost L-Ornithine is favored for its potential to enhance sleep quality by reducing ammonia levels in the body. It's a clean, straightforward supplement that aligns with health-conscious consumers due to its non-GMO and gluten-free certification. The supplement's commitment to purity and high user satisfaction rates make it one of my recommendations.

How We Chose These Items:

The selection of the best amino acids for sleep was a meticulous process grounded in comprehensive internet research and analysis of consumer feedback. Here's a snapshot of our method:

  • Extensive Product Analysis: We examined many products, focusing on those shown to support sleep through scientific research or have a longstanding tradition of use for this purpose.
  • Customer Reviews and Ratings: Real-world experiences and the satisfaction of current users on Amazon were paramount. We looked for products with many positive reviews and a low incidence of negative feedback.
  • Brand Credibility: We considered supplements from established brands with a solid track record in the industry, ensuring we recommend products from reputable sources.

These chosen amino acids have demonstrated efficacy in scientific studies and anecdotally amongst users, emphasizing safety and brand reliability.

Where to Buy

For those looking to enhance their well-being with amino acid supplements, you can easily find a selection of top-rated options on Amazon. Below are direct links to each amino acid supplement we've discussed for your convenience. As an Amazon Associate, we earn from qualifying purchases, which aids our mission to deliver valuable health and wellness information to you.

Purchasing through these links allows you to access quality supplements while supporting our efforts to provide insightful content about boosting your health naturally. It's essential to note that while we strive to provide accurate and up-to-date information, product availability and details can change, and it's always a good idea to check the current status on Amazon before making a purchase.

FAQs on Amino Acid Supplements for Sleep

How do amino acids like L-tryptophan and Glycine improve sleep quality? 
Amino acids such as L-tryptophan and Glycine are precursors to neurotransmitters that regulate sleep. L-tryptophan is converted into serotonin, which is then used to make melatonin, the hormone that controls sleep cycles. Glycine can lower body temperature, which signals the body it's time for sleep.

Can I take L-Theanine during the day, or will it tire me? 
L-Theanine promotes relaxation without causing drowsiness, so it's safe to take during the day. It is often used to help ease stress and improve focus.

Are there any side effects to taking amino acid supplements for sleep? 
Some amino acid supplements can have side effects, though they are typically mild. It's essential to follow the recommended dosages and consult a healthcare provider, especially if you take other medications.

How long does it take for amino acid supplements to affect sleep? 
The effects can vary by individual, but some people may notice improvements in sleep quality within a few days, while others may take a few weeks.

Can amino acid supplements be taken with other sleep medications? 
It's crucial to consult with a healthcare professional before combining supplements with other sleep medications to avoid interactions.


Our articles and recommendations are based on information (detailed product descriptions and customer reviews from their respective) taken directly from the official Amazon pages of the products mentioned. Additionally, the selection process was informed by internet research, including scientific studies on the efficacy of amino acids for sleep and the analysis of user testimonials.

Direct links to the amino acid supplements

These sources provide valuable insights into the benefits and applications of each supplement, ensuring that readers can make informed decisions about their health and sleep wellness.

After a thorough investigation into the scientific literature, several studies have been identified that discuss the effects of amino acids on sleep. Below are the references to the studies that were considered during this research:

  1. A systematic review and meta-analysis aimed to evaluate the efficacy of dietary supplements, including amino acids, on subjective sleep quality. The study concluded that supplementation with amino acids significantly improved subjective sleep quality. This meta-analysis provides evidence that amino acids could be a beneficial intervention for those seeking to improve their sleep quality​1​.
  2. Another study focused on the sleep-promoting and hypothermic effects of Glycine, one of the amino acids commonly associated with improved sleep. The study found that oral administration of Glycine in rats induced non-rapid eye movement (NREM) sleep and shortened NREM sleep latency while simultaneously decreasing core temperature, which is conducive to sleep. These findings suggest that Glycine promotes sleep via peripheral vasodilation by activating NMDA receptors in the suprachiasmatic nucleus​2​.
  3. A clinical trial investigated the effects of L-theanine on sleep quality in boys diagnosed with attention deficit hyperactivity disorder (ADHD). The trial demonstrated that 400 mg daily of L-theanine improved certain aspects of sleep quality, including sleep percentage and efficiency scores. The study proves that L-theanine, also an amino acid, can be a safe and beneficial aid in improving sleep, especially in populations with ADHD​3​.

These references highlight the potential benefits of amino acids such as Glycine and L-theanine in improving sleep quality. While more research, particularly in human trials, is warranted to understand the mechanisms and long-term effects fully, the current evidence supports the inclusion of these amino acids as part of a regimen for those struggling with sleep disturbances.